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. 2016 Jan 19;291(12):6304–6315. doi: 10.1074/jbc.M115.681031

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — PopD and PopB adopt mostly hexameric arrangements when assembled individually in membranes. A, TIRF microscopy image of PopD^BpyFL complexes in SLB. B, example of an intensity time trace obtained from a single membrane-assembled PopD^BpyFL complex. Single-molecule photobleaching events were evidenced by the stepwise decrease in the fluorescence intensity. C, quantification of photobleaching counts obtained from 100 time traces of single PopD^BpyFL complexes. D, TIRF microscopy image of PopB^BpyFL complexes in SLB. E, example of an intensity time trace obtained from a single membrane-assembled PopB^BpyFL complex. F, quantification of photobleaching counts obtained from 155 time traces of single PopB^BpyFL complexes. Filled dots in C and F represent how much of the total protein represented in the figure was present in each of the bleaching steps groups.