TABLE 1.

PCR primers used to generate the pUCPHD and pUCPHB vectors

The pUCPHB vector was generated using Gibson assembly combining the Cys-less P. aeruginosa PA01 popB gene amplified from plasmid pETDuet1-hispcrHpopB (14) using primers 1 and 2; a fragment from GHD containing the pcrG promoter and pcrH gene amplified using primers 3 and 4; and the pUCP18 vector linearized by double digestion with EcoRI and HindIII. Vectors containing the genes codifying for PopD^F223C or PopB^S164C were generated similarly using Gibson assembly with the linearized pUCP18 vector and fragments obtained from pUCPHD or pUCPHB with primers 3 and 7, 6 and 5, or 3 and 9, 8 and 2, respectively.

	Primer sequences
1	5′-TCCGATAACGCTTGAACGCGCCGGACTGCCCTA
2	5′-GCCAGTGCCAAGCTTTCAGATCGCTGCCGGTCGG
3	5′-CTATGACCATGATTACGAATTCTGACGCAGCGAAGCCTGC
4	5′-TCAAGCGTTATCGGATTCATATGTTCG
5	5′-CGACGGCCAGTGCCAAGCTTCAGACCACTCCGGCCGC
6	5′-CAGTCCTGCGTCCAGATGGCCAACGC
7	5′-CTGGACGCAGGACTGGATCACCGTGTTG
8	5′-CCAGAAGTGCGGTCTGGCAGCCAAAATC
9	5′-CAGACCGCACTTCTGGGATTCTTTCGC