FIGURE 1.

Characterization of recombinant apoA-I and reconstituted nascent HDL particles. Recombinant apoA-I (WT versus the indicated mutants) were purified from E. coli by nickel-nitrilotriacetic acid affinity chromatography, and nascent HDL particles were prepared using a modified cholate dialysis method at a molar ratio of 100:10:1 POPC/cholesterol/apoA-I. rHDL preparations were further purified using a Sephacryl-S300 size exclusion column. A, SDS-polyacrylamide gel analyses of purified recombinant apoA-I (WT and indicated point mutants). B, native polyacrylamide gel analyses of reconstituted nascent HDL containing recombinant WT apoA-I or the indicated point mutants.