FIGURE 3.

LCAT activities of nascent HDL. A, LCAT activities of reconstituted nascent HDL containing recombinant apoA-I (WT versus the different mutants indicated) were measured as described under “Experimental Procedures.” Note that Y166E/N and Y192F/L retain at least 80% of LCAT activities compared with WT, whereas P165A, Y166A/F, S167A, and D168A all exhibited significant reduction in the ability to activate LCAT compared with WT. B, Hanes-Woolf ([S]/V versus [S]) plots of nascent HDLs containing recombinant apoA-Is (WT, Y166A, and Y166E). Data shown represent the mean ± S.D. (error bars) of triplicate experiments. p values were determined by Student's t test.