TABLE 2.
Reaction kinetics of recombinant nascent HDL particles with LCAT
Apparent kinetic parameters for the interaction of recombinant human LCAT and the indicated rHDL were determined as described under “Experimental Procedures.” All values represent the mean ± S.D. of results from three independent determinations.
| HDL | Apparent Km | Apparent Vmax | Apparent Vmax/Km | Percentage of WT kcat (Vmax/Km) |
|---|---|---|---|---|
| μm | nmol/h/μg | nmol/h/μg/μm | % | |
| WT | 1.5 ± 0.3 | 14.9 ± 1.2 | 10.3 ± 1.4 | 100 |
| P165A | 2.1 ± 0.4 | 8.7 ± 0.2 | 4.3 ± 0.6 | 42 |
| Y166F | 10.0 ± 1.3 | 12.0 ± 0.8 | 1.2 ± 0.1 | 12 |
| Y166A | 2.3 ± 0.4 | 8.8 ± 0.4 | 3.9 ± 0.5 | 38 |
| Y166E | 2.0 ± 0.7 | 15.4 ± 1.8 | 7.9 ± 1.8 | 77 |
| Y166N | 1.5 ± 0.6 | 13.5 ± 2.0 | 9.4 ± 2.5 | 91 |
| S167A | 2.0 ± 0.8 | 5.6 ± 1.0 | 3.0 ± 0.6 | 29 |
| D168A | 4.5 ± 1.1 | 5.7 ± 0.8 | 1.3 ± 0.3 | 13 |
| Y192F | 2.4 ± 0.6 | 16.8 ± 0.5 | 7.2 ± 1.7 | 70 |
| Y192L | 2.0 ± 0.3 | 18.1 ± 0.9 | 8.9 ± 0.7 | 86 |