TABLE 3.
Equilibrium constants of recombinant human LCAT binding to WT and mutant recombinant apoA-I HDLs using surface plasmon resonance spectrometry
Dissociation constants (Kd) of binding human LCAT to rHDL were determined as described under “Experimental Procedures.” All values represent the mean ± S.D. of triplicate determinations.
| HDL | Kd | WT binding affinity |
|---|---|---|
| μm | % | |
| WT | 1.4 ± 0.3 | 100 |
| P165A | 3.9 ± 0.7 | 36 |
| Y166F | 6.0 ± 0.8 | 23 |
| Y166A | 6.4 ± 0.5 | 22 |
| Y166E | 1.3 ± 0.1 | 109 |
| Y166N | 1.0 ± 0.1 | 140 |
| Y166YNO2 | 30.0 ± 0.7 | 5 |
| S167A | 2.6 ± 0.4 | 54 |
| D168A | 7.5 ± 1.0 | 19 |
| Y192F | 1.4 ± 0.2 | 100 |