Table 4. Comparison of total capsid particle titer and vector genome titer.

Sample ID	ELISA titer (cp/ml)	ITR2 qPCR (vg/ml)	Free ITR (vg/ml)	Dot blot (vg/ml)
AAV8RSM	5.55 ± 2.79 E+11	2.65 ± 0.84 E+12	5.65 ± 1.75 E+11	5.65 ± 1.48 E+11
IC 5975	1.67 ± 0.37 E+13	5.01 ± 1.89 E+13	1.05 ± 0.38 E+13	9.63 ± 2.9 E+12

Total capsid particle (cp) titer was determined by an AAV8-specific ELISA, whereas vector genome titers were quantified by qPCR, using the ITR2 qPCR or the free-ITR qPCR, and dot blot. Values are shown as mean ± SD (n = 3–8).

AAV, adeno-associated virus; ITR, inverted terminal repeats; N.S., nonsignificant; RSM, reference standard materials; qPCR, quantitative polymerase chain reaction.