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. 2016 Mar 30;3:16015. doi: 10.1038/mtm.2016.15

Figure 2.

Figure 2

Distribution of adeno-associated virus (AAV)8 particles in cell lysates and culture media is dependent on the recombinant vector. Six groups (n = 3/group) of 293T/17 cells on 10 cm plates were transfected to produce AAV-FVIII, AAV-FIX, or AAV-PPCA. Cells and culture media were harvested on day 3 or 6 to determine whether AAV distribution was influenced by the recombinant vector. At days 3 and 6 post-transfection, the majority of AAV8 particles were located in the media for all three vector genomes, but this ratio changed more dramatically for FIX and PPCA than for FVIII. At day 3, 71% of the FVIII particles were in the media, while 65% of the FIX and 59% of the PPCA particles were in the media (the FVIII and PPCA groups were significantly different as measured by analysis of variance, P = 0.008). By day 6, 92% of FVIII, 90% of FIX and 88% of PPCA particles were in the media, indicating a greater shift in AAV particle distribution for FIX and, especially, PPCA.