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. 2016 Mar 30;3:16015. doi: 10.1038/mtm.2016.15

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Viral genome distribution in cell lysates and culture media tracks with particle distribution. Three 10-stack cell factories (CF1, CF2, and CF3) containing 293T/17 cells were transfected to produce adeno-associated virus (AAV)-FVIII. Cell lysates and media were harvested 6 days after transfection, and were processed separately for each cell factory. Quantitative polymerase chain reaction revealed that an average of 1.6 × 10¹³ ± 9.6 × 10¹¹ and 2.6 × 10¹² ± 3.4 × 10¹¹ total viral genomes were in the media and lysate, respectively, with an average of 85.9 ± 1.8% of FVIII genomes in the media across the three cell factories.