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. 2016 Mar 30;3:16015. doi: 10.1038/mtm.2016.15

Figure 4.

Figure 4

Adeno-associated virus (AAV) harvested at day 6 provides comparable expression to AAV harvested at day 3. Two preparations of three 10-stack cell factories of AAV-FVIII were purified after harvest of cell lysates and culture media at either day 3 or day 6 post-transfection. AAV-FVIII was injected into C57Bl/6 mice at a dose of 5 × 109, 2 × 1010, or 2 × 1011 viral genomes per mouse and plasma FVIII levels were analyzed 7 days postinjection (n = 4–5/group). Expression was comparable between AAV preparations (21.0 ± 7.6 ng/ml for D3 versus 15.0 ± 6.0 ng/ml for D6 for the low dose; 189.2 ± 83.9 ng/ml for D3 versus 113.7 ± 68.5 ng/ml for D6 for the medium dose; and 1,181.5 ± 371.5 ng/ml for D3 versus 1,019.2 ± 69.9 ng/ml for D6 at the high dose). Though AAV-FVIII harvested at day 3 post-transfection provides a slightly higher level of expression at all three time points, these differences were not statistically significant (P = 0.26, P = 0.19, and P = 0.37 at the low, medium, and high doses, respectively).