Figure 5.

Nigral pS6 immunoreactivity after doxycycline induction. Serine 235/236 phosphorylated S6 ribosomal protein (p-S6) immunostaining was performed 9 weeks following intra-striatal injection of 8.0 × 10⁹ vg of recombinant adeno-associated virus (rAAV)2/1-V16-hGDNF. Rats were fed with Dox-supplemented diet containing 0.025, 0.075, 0.2, or 1 g of Dox/kg or with unsupplemented food during 9 weeks. (a,b) Example of p-S6 immunoreactivity in substantia nigra pars compacta (SNc) of a rat treated by 0.2 g Dox /kg of diet (a) and an untreated rat (b). Scale bar: 2 mm (c) The p-S6 immunoreactivity is reported as the mean of the optical densities multiplied by the stained area measured on two to three slices at the level of SNc. n = 6 rats/Dox dose. **P < 0.01, ***; P < 0.001 versus no Dox group and ^#P < 0.05, ^###P < 0.001 versus enhanced green fluorescent protein group. GDNF, glial cell line-derived neurotrophic factor.