Figure 7.

Absence of astrocytic and microglial activation upon long-term recombinant adeno-associated virus (rAAV)2/1-V16 induction. Rats injected with rAAV2/1-V16-hGDNF (8.0 × 10⁹ vg) and treated with Dox diet at a concentration of 1 g/kg (d–f,j–l) or unsupplemented food (a–c,g–i) for 9 weeks. Brain sections were labeled with anti-IbaI (panels a–f) and anti-glial fibrillary acidic protein (panels g–l) antibodies. Pictures show the stainings of the injected (c,f,i,l) and intact (b,e,h,k) sides. Arrows indicate cells with the typical morphology of resting microglia (b–f) and astrocytes (h–l). Sections from two rats were labeled for each group. Scale bar: 500 µm for 2× magnification and 10 µm for 40× magnification. GDNF, glial cell line-derived neurotrophic factor.