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. 2016 Jun 17;5:136. Originally published 2016 Feb 4. [Version 2] doi: 10.12688/f1000research.7822.2

Figure 2.

Figure 2.

( A). Sequence and location of the 29 bp deletion in Gpr21 TAL 29bp KO mice. ( B). sequence and location of genotyping primers. ( C). Genotyping of Gpr21 TAL 29bp KO mice. Genomic DNA was generated from liver and BAT. PCR with genotyping primers amplified a 100 bp fragment from the genome of WT littermate mice and a 71 bp fragment from homozygous Gpr21 TAL 29bp KO mice. C: commercial mouse genomic DNA. L: 20 bp DNA ladder. ( D). No wildtype Gpr21 transcript were detected. qPCR analysis of Gpr21 gene in liver and BAT using primer/probe set that located in the 29 bp region and only detect wildtype Gpr21 transcript.