Figure 3. Engineered diversification and derivatization of antimycin (ANT) scaffolds by a promiscuous PKS/NRPS (adapted from Yan et al., 2013) ¹⁷.

Domain key: T = thioylation (e.g. acyl/peptidyl carrier protein ACP/PCP), C = condensation, A = adenylation, KR = ketoreductase, KS = ketosynthase, AT = acyl transacylase, TE = thioesterase. Green ball represents variable use of H or F-modified starting material. Red vs. blue star depicts unsaturated and carboxylated acyl substituent at C7, respectively. Orange triangle depicts variation in the alkyl chain of the C8 acyl substituent.