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. 2016 Mar 7;213(3):329–336. doi: 10.1084/jem.20151464

Figure 3.

Figure 3.

Sting−/− partially rescues perinatal lethality of G37S mice. (A) Quantitative RT-PCR analysis of a panel of mouse ISGs in WT or G37S embryos on Mavs−/− or Sting−/− or cGAS−/− background. Total RNA was isolated from primary MEFs (E13.5) of indicated genotype. (B) Mean ISG score of indicated genotypes. Data from A. (C) Gap junction cGAMP bioassay. As in Fig. 2 E, MEFs of indicated genotype were co-cultured with human BJ-1 cells for 18 h, with or without CBX or direct contact (indicated on the bottom). Quantitative RT-PCR analysis of IFN-β and IFIT1 indicates cGAMP activity in MEFs. (D) Mouse body weights. n = 4. (E) White-spotting phenotype in Rnaseh2aG37S/G37S Sting−/− viable adults. (F) Quantitative PCR analysis of mouse Line-1 5′ UTR and ORF2 DNA in WT or G37S embryos (isolated from E13.5 or E15.5; n = 3). Each dot represents an individual embryo. Mice were compared with littermate controls and with age-matched knock-out mice **, P < 0.01. Data are representative of at least two independent experiments (A–C), or pooled data from multiple animals (D–F). Error bars represent the SEM. Unpaired Student’s t test (F).