Fig. 1.

Comparison of UWMS MUC5b and MUC7 from SS patients and controls. (A) Mucins were detected using PAS stain for mucin glycosylation detection (PAS) and protein detected using Western blotting (WB) with antibodies directed to a nonglycosylated region of the protein. (B) Sialic acid was assessed by SNA (MUC5B) and MALII (MUC7) lectins. (C) Quantification of mucins used serially diluted purified mucin standards and the mucin glycan/protein proportion calculated individually and the mean calculated. Statistical significance is shown as * p ≤ .05, **** p ≤ .0001.