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. 2015 Dec 2;15(3):1060–1071. doi: 10.1074/mcp.M115.056226

Fig. 3.

Fig. 3.

Titration analysis of SNV and novel junction peptide discovery. Titration to determine the number of tandem MS process replicates needed to identify all variant peptides for iTRAQ (A) and label free (B) and novel junction peptides for iTRAQ (C) and label free (D) MS/MS. iTRAQ center A corresponds to process replicates 2, 3, 8, 10, 11, 13, and 14; center B replicates 4, 6, 9, 15, 16 and 18; and center C replicates 1, 5, 7, 12, and 17. Only label-free analysis from center E was used in this analysis (B, D). Basal and luminal tumor peptide identifications were pooled for titration analysis. Total list of SNV and novel junction peptides are included in Supplemental Tables 1–2.