Fig. 4.

Quantification of PON1 and APOC2 by immunoblot analysis and ELISA, respectively. (A) Relative quantification of PON1 in 14 subjects. For MS, the relative abundance of PON1 peptide was normalized to [¹⁵N]APOA1 peptide. For immunoblot analysis, APOA1 was used as loading control. (B) Pearson correlation between MS and immunoblot measurement for PON1. (C) APOC2 quantification by ELISA in all 60 cases and controls. The box plots show the distribution of the data (median, interquartile ranges), while the dots represent outliers. Concentration is expressed as ηg/μg protein. (D) Pearson correlation between MS and ELISA measurements for APOC2.