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High stringency washes effectively purify protein complexes for on-bead cross-linking. A, BS3- lanes represent one half of an xIP-MS experiment using 1 ml of cell lysate. The second half was resuspended in cross-linking buffer for on-bead cross-linking as described in text, and is shown in the BS3+ lanes. All lanes were resolved on the same gel at the same exposure; black lines indicated where lanes were cropped together for visibility.
