Skip to main content
. 2015 Nov 11;15(3):854–865. doi: 10.1074/mcp.M115.053082

Fig. 4.

Fig. 4.

xIP-MS suggests a model for human cohesin head interactions. A, LFQ analysis identifies canonical cohesin tetrameric subunits as interactors of SMC1A. Data is plotted as described previously. B, SMC1A and SMC3 interact at a near 1:1 stoichiometry as indicated by iBAQ values. C, Cross-links between SMC1A and SMC3 preferentially localize toward the head domains, indicated in green on the protein sequence bars. D, Homology modeling allows mapping of SMC1A and SMC3 cross-links onto models for the human proteins. SMC1A is colored light gray, and SMC3 is colored in dark gray. Cross-links below 34 Å are shown in blue, and cross-links above 34 Å are shown in red. The silhouette represents the surface of the SMC3 dimer from the PDB: 4ux3 crystal structure used for aligning the SMC1A and SMC3 models.