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. 2015 Nov 11;15(3):854–865. doi: 10.1074/mcp.M115.053082

Fig. 6.

Fig. 6.

xIP-MS reveals S-phase independent MCM subcomplex topologies. A, MCM2/4/6/7, MCM10, and MCMBP are observed as interactors with MCM6 in asynchronous cells. B, Cross-linking analysis reveals interprotein contacts within soluble MCM complexes in asynchronous cells. C, Similarly, MCM2/4/6/7, MCM10, and MCMBP are observed as interactors with MCM6 in S-phase blocked cells. D, Cross-linking analysis reveals a similar topology in soluble S-phase MCM complexes. E, Relative stoichiometries to the bait as indicated by iBAQ values show MCM subunit stoichiometries do not change substantially when cells are blocked in S-phase. F, Venn diagram indicates substantial overlap of cross-linked sites between MCM subunits after S-phase block.