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. 2015 Dec 29;15(3):878–891. doi: 10.1074/mcp.M115.053207

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version free via Creative Commons CC-BY license.

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Fig. 4. — NuRD associates with the MLL methyltransferase complex regulatory subunit Wdr5. A, BN-PAGE migration profile of Wdr5 showing correlation to the NuRD reference profiles of Mta2 and Hdac1. B, Linear regression plot of normalized iBAQ relative to Mta2 for Wdr5, illustrating the comigration trend. C, Western blotting for NuRD subunits Mta2 (FLAG), Hdac2, Chd4, and Rbbp4 following Wdr5 IP. D, An ESC whole cell lysate was subject to size exclusion chromatography. Twenty-eight fractions were collected (3B5–3D8) and analyzed by Western blotting for Mta2-FLAG, Hdac2, Sall4, and Wdr5.