Skip to main content
. 2015 Dec 29;15(3):878–891. doi: 10.1074/mcp.M115.053207

Fig. 5.

Fig. 5.

Sall4 associates with the high-mass, but not with the low-mass NuRD entity. A, Migration profiles for Mta2, Hdac1, (NuRD reference profiles) and Cdk2ap1. B, Migration profiles for Mta2, Hdac1, and Sall4, which outline comigration of Sall4 with the high-mass NuRD entity. Mta2 and Hdac1 provide a reference profile of the NuRD complex. C, Sall4 Western blotting of FLAG immunoprecipitates from Mta2-FTAP cells after BN-PAGE separation. The immunoprecipiate was divided in two aliquots of which one was denatured (D) and the other left native (N) prior to BN-PAGE. Molecular weight markers are indicated in kDa. D, Suz12 migration profile in the NuRD region. Mta2 and Hdac1 are references for the NuRD migration profile. E, Schematic diagram of the serial immunoprecipitation procedure. FLAG was isolated from the whole cell lysate. The immunoprecipitate provided the input for the subsequent Sall4 purification. The lysate, both immunoprecipitates (IP) and both flow-throughs (FT) were subject to Western blotting. F, Successive FLAG (Mta2) and Sall4 IPs were performed, first on a whole cell lysate from Mta2-FTAP cells and subsequently on the eluate from the first IP. The input lysate, immunoprecipitates (IP) and flow-throughs (FT) were probed for FLAG, Sall4, Hdac2, and Rbbp4.