Fig. 1.

Global identification of Kme1 sites using SILAC-based proteomics and Kme1 immunoaffinity enrichment. A, Western blot analysis of SMYD2 or β-actin in parental KYSE-150 cells infected with lentivirus expressing either control shRNA (shControl) or shRNA targeted against SMYD2 (shSMYD2). B, Western blot analysis of p53-K370me1, total p53, SMYD2, and β-actin following treatment of KYSE-150 cells overexpressing SMYD2 with either DMSO or LLY-507. C, Overview of the experimental approach used for SILAC-based peptide labeling and enrichment of lysine mono-methylated peptides in this study. D, Comparison of current and previously published datasets in terms of the number of Kme1 sites (black) and proteins with reported Kme1 sites (red). E, Venn diagram illustrating the number of reproducible Kme1 sites in parental cells and in SMYD2-overexpressing cells.