Fig. 3.

SMYD2 knockdown down-regulates the abundance of Kme1 sites commonly identified in proteomic experiments and across a variety of cancer cell lines, including BTF3-K2 and PDAP1-K126. A, Number of Kme1 sites (red) and mono-methylated proteins (black) identified in the indicated cell lines. Only the 273 reproducible Kme1 sites in the parental KYSE-150 cell line are represented. B, The number of overlapping Kme1 sites identified in the indicated proteomic datasets among with the cell lines that were profiled in the respective studies. Specific Kme1 sites are listed in Supplemental Table S3. C, Kme1 sites (and their sequences) that were identified in at least four of the seven cell lines profiles to date and their normalized SILAC peptide ratio in response to SMYD2 knockdown in parental KYSE-150 cells. * = p value ≤ 0.05 relative to AKAP13-K1670me1. D, Bioluminescence assays monitoring the production of SAH following incubation of 100 nm of protein substrate with the indicated concentrations of SMYD2. E, MS1 peak quantification of the indicated Kme1 peptide following biochemical methylation reactions using full-length recombinant BTF3 or PDAP1 with the indicated concentrations of SMYD2. 1, Cao et al., 2013; 2, Wu et al., 2014.