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. 2016 Jan 10;15(3):892–905. doi: 10.1074/mcp.M115.053280

Fig. 5.

Fig. 5.

AHNAK and AHNAK2 are mono-methylated at multiple sites by SMYD2. A, Changes in the number of Kme1 sites per protein identified in parental KYSE-150 cells and in SMYD2-overexpressing cells. Only reproducible Kme1 sites (i.e. identified in two of three experimental replicates) are included. AHNAK and AHNAK2 are highlighted in red. B, Protein sequence alignment of select Kme1 sites in AHNAK and AHNAK2 that were down-regulated by SMYD2 knockdown. C, Protein domain architecture of AHNAK and AHNAK2 and the positioning of the identified Kme1 sites within a representative consensus central repeat unit (CRU) of each protein. The asterisk indicates that this site ambiguously maps to AHNAK as well. E, MS1 peak quantification of mono-methyl peptide products from biochemical methylation reactions of AHNAK2–4CRU with the indicated concentration of recombinant SMYD2. Abbreviations: me0, unmodified peptide; me1, mono-methylated peptide; 1Me, single mono-methylation site; 2Me, double mono-methylation site.