Fig. 7.

Prenucleosomal CENP-A-Bound H4 is N-terminally acetylated and lacks H4K20 methylation. A, Relative abundances of methylated and acetylated PTM-forms of AspN-generated H4 S1-R23 amino-terminal tail peptide found in the Mitotic Prenucleosomal sample. B, Comparison of H4 tail PTM forms from Mitotic Prenucleosomal CENP-A-LAP versus Asynchronous Nucleosomal H4 forms observed in this study shows H4 molecules bound to prenucleosomal CENP-A are enriched in forms that lack H4K20 methylation and contain α-N-terminal acetylation as well as acetylations at K5 and K12.