Table 1. The primer sequences for real-time PCR.
Genes | GenBank numbers | Primer sequence | Product size (bp) |
---|---|---|---|
β-Actin | NM-031144.2 | Forward 5′-GGAGATTACTGCCCTGGCTCTA-3′ Reverse 5′-GACTCATCGTACTCCTGCTTGCTG-3′ | 150 |
IGF-I | NM 001082478.1 | Forward 5′-GCACTCTGCTTGCTCACCTTTA-3′ Reverse 5′-TCCGAATGCTGGAGCCATA-3′ | 148 |
IGF-IR | NM 052807.2 | Forward 5′-GGTCTCTAAGGCCAGAGGTGGA-3′ Reverse 5′-GACGAACTTGTTGGCATTGAGGTA-3′ | 122 |