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Effects of DHA treatment on MITF and tyrosinase levels. B16F10 cells were cultured with 25 μmol/L of DHA for 24–72 h. Whole-cell lysates were subjected to Western blot analysis with antibodies against (A) MITF and tyrosinase or (B) TRP1. Equal protein loading was verified by reactivity with an anti-actin antibody. Band intensity relative to the control was determined by densitometric analysis. Values are expressed as the mean±SD (n=3). bP<0.05 compared to α-MSH-treated control.
