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. 2016 Jan 7;26(5):482–492. doi: 10.1093/glycob/cww001

Table I.

Summary of oligosaccharide analysis of the rhATs produced by CHO cells

Samplesa Relative composition of monosaccharides
Structured
Fucose GlcNAcb Mannose Galactose Sialic acidc
phAT 0.03 4.0 2.3 1.9 2.0
rhAT-Manα n.d. 2.0 5.3 n.d. n.d. I
rhAT-Manβ n.d. 2.0 5.4 n.d. n.d. I
rhAT-Comα n.d. 4.0 1.9 2.1 2.3 II, III
rhAT-Comβ n.d. 4.0 2.2 1.8 1.8 II, III

graphic file with name cww00107.jpg

Sialic acid (closed square), galactose (open diamond), GlcNAc (closed circle) and mannose (open square).

n.d., not detected.

aThe purified phAT, rhAT-Manα, rhAT-Manβ, rhAT-Comα and rhAT-Comβ were employed as samples.

bMolar ratios calculated versus GlcNAc composition.

cMol sugar/oligosaccharide chain.

dSchematic of the major oligosaccharide structures detected by MALDI-TOF MS are described above.