Fig. 1.

CLIPA2 kd mosquitoes are more tolerant and resistant to E. coli injections. A. gambiae adult female mosquitoes were injected with E. coli strain OP-50 (OD₆₀₀ = 0.4) 4 days after silencing with dsLacZ (control), dsTEP1 and dsCLIPA2. a, b Dead mosquitoes were counted daily over a period of 9 days after bacterial challenge. Percent survival was calculated using the Kaplan-Meier method for 2 independent representative experiments. Statistical significance was calculated using the log-rank test. Survival curves were considered to be significantly different if p < 0.05. c Two days after injection of E. coli (OD₆₀₀ = 0.4), 5 batches of 8 mosquitoes each per indicated genotype were ground in PBS, and live bacterial counts were determined by plating serial dilutions on LB plates supplemented with ampicillin. Graphs represent mean CFU values per mosquito with their standard errors. Statistical significance was calculated using the Mann-Whitney test, and asterisks indicate p < 0.05. Data shown are from 2 independent biological experiments.