Table 2.
Proteins interacting with HA-tagged GlNR1 in vivo. G. lamblia WBC6 was transformed with GlNR1-3xHA. Crude extracts were affinity-purified using anti-HA-antibodies immobilised on beads followed by mass spectrometry. A control experiment was performed with crude extract from WBC6 expressing no recombinant protein. Only hits with highest stringency, namely, minimal mascot score of 95 for peptide probability, a protein probability of 95% and a minimum of 2 unique peptides per protein, are shown.
| Name | Accession-N° | Molecular weight (kDa) | Unique peptides (N°) |
|---|---|---|---|
| Axoneme-associated protein GASP-180 | GL50803_137716 | 175 | 4 |
| Fructose-bisphosphate aldolase | GL50803_11043 | 35 | 3 |
| GlNR2 (Fd-NR1) | GL50803_6175 | 31 | 2 |
| Hypothetical protein | GL50803_9183 | 214 | 2 |
| TCP1-chaperon-subunit gamma | GL50803_17411 | 62 | 2 |
| Phosphoglycerate kinase | GL50803_90872 | 44 | 2 |
| Arginyl-tRNA-synthetase | GL50803_10521 | 70 | 2 |
| Vacuolar ATP-synthase catalytic subunit A | GL50803_7532 | 72 | 2 |
| Malate dehydrogenase | GL50803_3331 | 35 | 2 |