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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: Biochem Pharmacol. 2016 Mar 2;106:94–103. doi: 10.1016/j.bcp.2016.02.020

Fig. 5. TCDD-stimulated AHR/ARNT/p300 recruitment and histone H3 acetylation at an AHR binding site in the LAT1 gene.

Fig. 5

A-E, MCF-7 cells were treated with DMSO or 10 nM TCDD for 45 minutes. Cells were then subjected to ChIP with non-specific IgG (IgG), or AHR, ARNT, p300, acetylated histone H3 (lysine 9 or 14) targeting antibodies, followed by qPCR amplification of the AHR binding site in the LAT1 (A-C) or CYP1B1 (D & E) genes. Significant increases in AHR, ARNT, or p300 binding to AHR binding sites in the LAT1 (A-C) or CYP1B1 (D & E) gene by TCDD are indicated by *, P < 0.05, or **, P < 0.01. C, Significant increases in the acetylation of lysine 9 or 14 in histone H3 at the AHR binding site in the LAT1 gene by TCDD are indicated by #, P < 0.05. Data shown are the means ± S.E. of three independent experiments.