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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: Biochem Pharmacol. 2016 Mar 2;106:94–103. doi: 10.1016/j.bcp.2016.02.020

Fig. 6. Endogenous regulation of LAT1 by AHR in MCF-7 and MDA-MB-231 cells.

Fig. 6

A-D, MCF-7 (A & B) or MDA-MB-231 (C & D) cells were transfected with control (cRNAi) or AHR (AHRi) siRNA for 72 or 48 hrs, respectively. A & C, Total cellular protein was then isolated and subjected to Western blot analysis. Blots were then probed with the indicated antibodies. Relative levels of AHR or LAT1 protein was expressed as a ratio of AHR/GAPDH or LAT1/GAPDH, respectively. *, P < 0.05; **, P >0.01. B & D, RT-qPCR analyses of LAT1 mRNA levels in MCF-7 (B) or MDA-MB-231 (D) cells transfected with cRNAi or AHRi. GAPDH mRNA levels were used to normalize samples. *, P < 0.05. A-D, Data shown are the means ± S.E. of three independent experiments.