Figure 3.

(A) Scheme of the nanoconstruct of the thymine modified paramagnetic particles (PMPs), which were bound with (AAA)₁₀ODN-SH based on their complementarity. On the thiolated ends of oligonucleotides the AuNPs were tied to modified liposome with encapsulated fluorescence label. On the complementary domain of (AAA)₁₀ODN-SH the as-N-myc sequence was hybridized for binding to N-myc gene; (B) Spectrophotometric analysis of relative ssDNA concentration and (C) electrochemical analysis (square wave voltammetry) of relative CA peak height during hybridization of: (I.) 50 µg·mL⁻¹ (AAA)₁₀ODN-SH isolated by (II.) thymine labelled MPs and (III.) attached 50 µg·mL⁻¹ elongated as-N-myc sequence for (IV) binding of 50 µg·mL⁻¹ N-myc gene. During the individual hybridization steps, samples were washed with phosphate buffer; (D) Fluorescence spectra of isolated drugs (ellipticine, doxorubicin, or etoposide, all in concentrations of 160 µg·mL⁻¹). Excitation wavelengths for ellipticine, doxorubicin and etoposide were 420, 480 and 250 nm, respectively; (E) Concentration yield of encapsulated ellipticine, doxorubicin, or etoposide (160 µg·mL⁻¹) in the liposome after nanoconstruct isolation.