Figure 3.

QSN-10c increased apoptosis in K562 cells and HUVECs. PI staining (A–B) and FITC-conjugated Annexin V/PI staining (C-D) were conducted to evaluate the cell apoptosis induced by QSN-10c. (A) Representative patterns of flowcytometric distribution of K562 cells treated with QSN-10c for different time. M1 marks subG₁ apoptosic cells. (B) Quantitative comparison of the numbers of subG₁ phase in different groups. (C) Representative patterns of flowcytometric distribution of K562 cells. Dot plot was divided into a quadrant: upper left, necrotic cells; upper right, late apoptotic cells; lower left, living cells; and lower right, early apoptotic cells. (D) Graphs demonstrate the sum of cells in early and late apoptosis of K562 cells. (E) Representative patterns of flowcytometric distribution of HUVEC. Cells receiving only DMSO (0.1%) served as a vehicle control. Data were expressed as mean±SD. n=3. ^cP<0.01 vs control.