Figure 7.

Effects of QSN-10c on VEGF expression in HUVECs. (A) Electrophoresis bands expressed with VEGF and internal standard GAPDH mRNA in various groups treated with different concentrations of thalidomide and QSN-10c. The upper bands were corresponded to VEGF, and the lower to GAPDH. (B) Relative VEGF mRNA levels were quantified by integral optical density analysis of the bands from (A) and then normalization to GAPDH in HUVECs. Cells received thalidomide as positive control. Data are expressed as percentages of the vehicle control (100%) in mean±SD from three independent experiments. ^bP<0.05, ^cP<0.01 compared with control. (C) HUVECs were treated with QSN-10c for 24 h. Cell extract was prepared and subjected to Western blot using anti-VEGF antibody. β-Actin served as a loading control. QSN-10c decreased VEGF protein expression in HUVECs in a dose dependent manner as detected by Western blot.