Table 2. Associations between MKK7 rare polymorphisms and lung cancer risk.
| MKK7 SNPs | Discovery set | Validation set I | Validation set II | Pooled analysis b | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Case n (%) | Control n (%) | Adjusted OR (95% CI) a | Case n (%) | Control n (%) | Adjusted OR (95% CI) a | Case n (%) | Control n (%) | Adjusted OR (95% CI) a | Case n (%) | Control n (%) | Adjusted OR (95% CI) a | |
| p. Glu116Lys (rs28395770G>A) | 1559 | 1679 | 1949 | 1957 | 1508 | 1545 | 5016 | 5181 | ||||
| Codominant model c | 4417.3 | 5315.2 | 4037.6 | 13824.3 | ||||||||
| Glu/Glu | 1437(92.2) | 1638(97.6) | 1.00 (ref.) | 1786(91.6) | 1908(97.5) | 1.00 (ref.) | 1373(91.0) | 1495(96.8) | 1.00 (ref.) | 4596(91.6) | 5041(97.3) | 1.00 (ref.) |
| Lys/Glu | 111(7.1) | 38(2.3) | 3.33(2.29–4.86) | 140(7.2) | 42(2.1) | 3.53(2.48–5.01) | 119(7.9) | 45(2.9) | 2.83(1.97–4.05) | 370(7.4) | 125(2.4) | 3.23(2.62–3.98) |
| Lys/Lys | 11(0.7) | 3(0.1) | 3.94(1.09–14.3) | 23(1.2) | 7(0.4) | 3.52(1.50–8.26) | 16(1.1) | 5(0.3) | 3.26(1.16–9.14) | 50(1.0) | 15(0.3) | 3.75(2.09–6.71) |
| Fisher test P value | 4.12×10−12 | 7.99×10−16 | 8.98×10−11 | 1.09×10−36 | ||||||||
| Additive model c | 4416.9 | 5317.9 | 4037.5 | 13829.6 | ||||||||
| Lys/Lys vs. Lys/Glu vs. Glu/Glu | 3.00(2.14–4.21) | 2.85(2.14–3.81) | 2.49(1.83–3.38) | 2.80(2.34–3.34) | ||||||||
| Dominant model c | 4415.3 | 5313.2 | 4035.7 | 13822.5 | ||||||||
| Glu/Glu | 1437(92.2) | 1638(97.6) | 1.00 (ref.) | 1786(91.6) | 1908(97.5) | 1.00 (ref.) | 1373(91.0) | 1495(96.8) | 1.00 (ref.) | 4596(91.6) | 5041(97.3) | 1.00 (ref.) |
| Lys/Lys+Lys/Glu | 122(7.8) | 41(2.4) | 3.38(2.35–4.85) | 163(8.4) | 49(2.5) | 3.52(2.54–4.89) | 135(9.0) | 50(3.2) | 2.87(2.04–4.04) | 420(8.4) | 140(2.7) | 3.29(2.70–4.01) |
| p.Asn118Ser (rs56316660A>G) | ||||||||||||
| Codominant model c | 4464.1 | 5381.6 | 4077.2 | 13982.3 | ||||||||
| Asn/Asn | 1535(98.5) | 1664(99.1) | 1.00 (ref.) | 1921(98.6) | 1933(98.8) | 1.00 (ref.) | 1490(98.8) | 1532(99.2) | 1.00 (ref.) | 4946(98.6) | 5129(99.0) | 1.00 (ref.) |
| Ser/Asn | 19(1.2) | 12(0.7) | 1.65(0.79–3.41) | 22(1.1) | 19(1.0) | 1.23(0.66–2.30) | 15(1.0) | 11(0.7) | 1.56(0.70–3.48) | 56(1.2) | 42(0.8) | 1.39(0.93–2.08) |
| Ser/Ser | 5(0.3) | 3(0.2) | 2.24(0.53–9.47) | 6(0.3) | 5(0.2) | 1.13(0.34–3.75) | 3(0.2) | 2(0.1) | 1.44(0.23–9.14) | 14(0.3) | 10(0.2) | 1.59(0.70–3.60) |
| Fisher test P value | 0.224 | 0.813 | 0.612 | 0.190 | ||||||||
| Additive model c | 4462.1 | 5379.7 | 4075.4 | 13980.4 | ||||||||
| Ser/Ser vs. Ser/Asn vs. Asn/Asn | 1.57(0.94–2.63) | 1.14(0.74–1.77) | 1.40(0.76–2.58) | 1.32(0.98–1.77) | ||||||||
| Dominant model c | 4462.2 | 5379.6 | 4075.2 | 13980.4 | ||||||||
| Asn/Asn | 1535(98.5) | 1664(99.1) | 1.00 (ref.) | 1921(98.6) | 1933(98.8) | 1.00 (ref.) | 1490(98.8) | 1532(99.2) | 1.00 (ref.) | 4946(98.6) | 5129(99.0) | 1.00 (ref.) |
| Ser/Ser+Ser/Asn | 24(1.5) | 15(0.9) | 1.76(0.91–3.37) | 28(1.4) | 24(1.2) | 1.21(0.70–2.11) | 18(1.2) | 13(0.8) | 1.54(0.74–3.22) | 70(1.5) | 52(1.0) | 1.43(0.98–2.05) |
| p.Arg138Cys (rs56106612C>T) | ||||||||||||
| Codominant model c | 4464.3 | 5381.3 | 4077.7 | 13982.6 | ||||||||
| Arg/Arg | 1539(98.7) | 1668(99.3) | 1.00 (ref.) | 1927(98.9) | 1940(99.1) | 1.00 (ref.) | 1491(98.9) | 1532(99.1) | 1.00 (ref.) | 4957(98.8) | 5140(99.2) | 1.00 (ref.) |
| Cys/Arg | 15(1.0) | 9(0.5) | 1.70(0.74–3.91) | 18(0.9) | 14(0.7) | 1.27(0.63–2.56) | 15(1.0) | 12(0.8) | 1.18(0.54–2.56) | 48(1.0) | 35(0.7) | 1.35(0.87–2.10) |
| Cys/Cys | 5(0.3) | 2(0.1) | 2.42(0.47–12.6) | 4(0.2) | 3(0.2) | 1.54(0.34–6.93) | 2(0.1) | 1(0.1) | 2.75(0.23–32.8) | 11(0.2) | 6(0.1) | 1.89(0.70–5.16) |
| Fisher test P value | 0.192 | 0.688 | 0.627 | 0.131 | ||||||||
| Additive model c | 4462.4 | 5379.3 | 4075.9 | 13980.7 | ||||||||
| Cys/Cys vs. Cys/Arg vs. Arg/Arg | 1.63(0.90–2.94) | 1.25(0.75–2.10) | 1.30(0.68–2.50) | 1.36(0.98–1.90) | ||||||||
| Dominant model c | 4462.5 | 5379.4 | 4076.2 | 13981.0 | ||||||||
| Arg/Arg | 1539(98.7) | 1668(99.3) | 1.00 (ref.) | 1927(98.9) | 1940(99.1) | 1.00 (ref.) | 1491(98.9) | 1532(99.1) | 1.00 (ref.) | 4957(98.8) | 5140(99.2) | 1.00 (ref.) |
| Cys/Cys+Cys/Arg | 20(1.3) | 11(0.6) | 1.83(0.87–3.85) | 22(1.1) | 17(0.9) | 1.31(0.69–2.49) | 17(1.1) | 13(0.9) | 1.28(0.61–2.68) | 59(1.2) | 41(0.8) | 1.43(0.96–2.14) |
| p.Ala195Thr (rs55800262G>A) | ||||||||||||
| Codominant model c | 4464.1 | 5381.7 | 4077.2 | 13982.6 | ||||||||
| Ala/Ala | 1537(98.6) | 1666(99.2) | 1.00 (ref.) | 1930(99.0) | 1941(99.2) | 1.00 (ref.) | 1487(98.6) | 1529(98.9) | 1.00 (ref.) | 4954(98.8) | 5136(99.1) | 1.00 (ref.) |
| Thr/Ala | 18(1.2) | 11(0.7) | 1.75(0.82–3.73) | 13(0.7) | 12(0.6) | 1.16(0.52–2.56) | 16(1.1) | 12(0.8) | 1.51(0.70–3.27) | 47(0.9) | 35(0.7) | 1.43(0.92–2.24) |
| Thr/Thr | 4(0.3) | 2(0.1) | 2.18(0.40–12.0) | 6(0.3) | 4(0.2) | 1.39(0.39–4.97) | 5(0.3) | 4(0.3) | 1.47(0.37–5.90) | 15(0.3) | 10(0.2) | 1.49(0.67–3.35) |
| Fisher test P value | 0.198 | 0.762 | 0.703 | 0.189 | ||||||||
| Additive model c | 4462.2 | 5379.7 | 4075.4 | 13980.9 | ||||||||
| Thr/Thr vs. Thr/Ala vs. Ala/Ala | 1.63(0.92–2.89) | 1.17(0.71–1.93) | 1.34(0.80–2.25) | 1.32(0.97–1.78) | ||||||||
| Dominant model c | 4462.2 | 5379.7 | 4075.2 | 13980.6 | ||||||||
| Ala/Ala | 1537(98.6) | 1666(99.2) | 1.00 (ref.) | 1930(99.0) | 1941(99.2) | 1.00 (ref.) | 1487(98.6) | 1529(98.9) | 1.00 (ref.) | 4954(98.8) | 5136(99.1) | 1.00 (ref.) |
| Thr/Thr+Thr/Ala | 22(1.5) | 13(0.8) | 1.82(0.91–3.63) | 19(1.0) | 16(0.8) | 1.22(0.62–2.39) | 21(1.4) | 16(1.1) | 1.50(0.76–2.95) | 62(1.2) | 45(0.9) | 1.45(0.98–2.14) |
| p.Leu259Phe (rs1053566 C>T) | ||||||||||||
| Codominant model c | 4464.2 | 5381.7 | 4077.4 | 13982.4 | ||||||||
| Leu/Leu | 1543(99.0) | 1670(99.4) | 1.00 (ref.) | 1936(99.3) | 1946(99.4) | 1.00 (ref.) | 1491(98.9) | 1532(99.2) | 1.00 (ref.) | 4970(99.1) | 5148(99.3) | 1.00 (ref.) |
| Phe/Leu | 10(0.6) | 6(0.4) | 1.89(0.68–5.25) | 9(0.5) | 8(0.4) | 1.13(0.43–2.94) | 14(0.9) | 11(0.7) | 1.44(0.63–3.28) | 33(0.7) | 25(0.5) | 1.45(0.85–2.44) |
| Phe/Phe | 6 (0.4) | 3(0.2) | 2.28(0.57–9.18) | 4(0.2) | 3(0.2) | 1.52(0.33–6.92) | 3(0.2) | 2(0.1) | 1.82(0.28–11.7) | 13(0.3) | 8(0.2) | 1.83(0.75–4.47) |
| Fisher test P value | 0.300 | 0.847 | 0.679 | 0.250 | ||||||||
| Additive model c | 4462.3 | 5379.7 | 4075.4 | 13980.4 | ||||||||
| Phe/Phe vs. Phe/Leu vs. Leu/Leu | 1.63(0.91–2.91) | 1.19(0.66–2.16) | 1.39(0.99–1.96) | |||||||||
| Dominant model c | 4462.2 | 5379.8 | 4075.5 | 13980.6 | ||||||||
| Leu/Leu | 1543(99.0) | 1670(99.4) | 1.00 (ref.) | 1936(99.3) | 1946(99.4) | 1.00 (ref.) | 1491(98.9) | 1532(99.2) | 1.00 (ref.) | 4970(99.1) | 5148(99.3) | 1.00 (ref.) |
| Phe/Phe+Phe/Leu | 16(1.0) | 9(0.6) | 2.02(0.89–4.61) | 13(0.7) | 11(0.6) | 1.23(0.54–2.76) | 17(1.1) | 13(0.8) | 1.50(0.70–3.18) | 44(1.0) | 33(0.7) | 1.54(0.98–2.42) |
a Data were calculated by exact logistic regression adjusted for selected surrounding factors.
b The data comprised the discovery set and two validation sets.
c Akaike information criterion (AIC) value.