Extended Data Table 1.
| Figure | Groups (Ns refer to animals in behavior and c-fos experiments and cells in electrophysiology experiments) | Statistical Analysis |
|---|---|---|
| 1d (RTPP) | eGFP control (n = 8) ChR2 + saline (n = 10) ChR2 + NBQX (n = 8) ChR2 + D1R antagonist (n = 8) ChR2 + D2R antagonist (n = 8) |
One-way ANOVA: F(4, 37) = 29.61, p < 0.0001 Post-hoc Tukey's test: ChR2 + saline vs. GFP control, p < 0.001 ChR2 + saline vs. ChR2 + NBQX, p < 0.001 ChR2 + saline vs. ChR2 + D1R antagonist, p = 0.98 ChR2 + saline vs. ChR2 + D2R antagonist, p = 0.58 |
| 2c (c-fos) | Morphine + saline (n = 5) Morphine + naloxone (n = 6) |
Mann-Whitney U-test Morphine + saline vs. Morphine + naloxone, p < 0.01 |
| 2d (Behavioral signs) | eGFP control (n = 9) ArchT silencing (n = 10) |
Mann-Whitney U-test Jump: GFP vs. Arch, p < 0.001 Rearing: GFP vs. Arch, p < 0.01 Tremor: GFP vs. Arch, p < 0.001 |
| 2e (CPA) | eGFP control (n = 9) ArchT silencing (n = 10) |
Mann-Whitney U-test 1d CPA: GFP vs. Arch, p < 0.05 7d CPA: GFP vs. Arch, p < 0.05 |
| 2f (hM4Di silencing current) | Baseline (n = 5) CNO (n = 5) |
Wilcoxon signed-rank test Baseline vs. CNO, p < 0.05 |
| 2g (Spontaneous withdrawal) | eGFP + CNO (n = 8) hM4D + CNO (n = 8) hM4D + saline (n = 8) |
One-way ANOVA: F(2, 21) = 7.4, p < 0.01 Post-hoc Tukey's test: hM4D + CNO vs. GFP + CNO, p < 0.05 hM4D + CNO vs. hM4D + saline, p < 0.01 hM4D + saline vs. GFP + CNO, p = 0.55 |
| 2h (Locomotion) | eGFP control (n = 9) ArchT silencing (n = 9) |
Wilcoxon signed-rank test Saline: GFP vs. Arch, p = 0.57 Morphine: GFP vs. Arch, p = 0.5 |
| 3c (AN ratio) | Saline D1 (n = 14) Saline D2 (n = 13) Morphine D1 (n = 14) Morphine D2 (n = 16) |
Two-way factorial ANOVA Group (saline, morphine) × Cell (D1, D2) Group: F(1,53) = 17.01, p < 0.001 Cell: F(1,53) = 9.18, p < 0.01 Group × Cell: F(1,53) = 12.58, p < 0.001 Post-hoc Tukey's test: saline D1 vs morphine D1, p = 0.97 saline D2 vs morphine D2, p < 0.001 |
| 3e (Rectification index) | Saline D1 (n = 8) Saline D2 (n = 7) Morphine D1 (n = 9) Morphine D2 (n = 10) |
Two-way factorial ANOVA Group (saline, morphine) × Cell (D1, D2) Group: F(1,30) = 6.65, p < 0.05 Cell: F(1,30) = 4.32, p < 0.05 Group × Cell: F(1,30) = 9.87, p < 0.01 Post-hoc Tukey's test: saline D1 vs morphine D1, p = 0.97 saline D2 vs morphine D2, p < 0.01 |
| 4c (AN ratio) | Morphine D1 (n = 14) Morphine D2 (n = 16) Morphine + 1Hz, D1 (n = 14) Morphine + 1Hz, D2 (n = 17) |
Two-way factorial ANOVA Group (morphine, morphine + 1Hz) × Cell (D1, D2) Group: F(1,57) = 21.59, p < 0.0001 Cell: F(1,57) = 11.57, p < 0.01 Group × Cell: F(1,57) = 13.86, p < 0.001 Post-hoc Tukey's test: Morphine D1 vs morphine 1Hz D1, p = 0.97 Morphine D2 vs morphine 1 Hz D2, p< 0.001 |
| 4e (Rectification index) | morphine D1 (n = 9) morphine D2 (n = 10) depotentiation D1 (n = 7) depotentiation D2 (n = 10) |
Two-way factorial ANOVA Group (morphine, morphine 1Hz) × Cell (D1, D2) Group: F(1,32) = 5.12, p < 0.05 Cell: F(1,32) = 8.23, p < 0.01 Group × Cell: F(1,32) = 4.3, p < 0.05 Post-hoc Tukey's test: saline D1 vs morphine D1, p = 0.92 saline D2 vs morphine D2, p < 0.05 |
| 4g (Behavioral signs) | eGFP control (n = 8) ChR2 depotentiation (n = 8) |
Mann-Whitney U-test Jump: GFP vs. ChR2, p < 0.01 Rearing: GFP vs. ChR2, p < 0.01 Tremor: GFP vs. ChR2, p < 0.01 |
| 4h (CPA) | eGFP control (n = 8) ChR2 depotentiation (n = 8) |
Mann-Whitney U-test 1d CPA: GFP vs. ChR2, p < 0.05 7d CPA: GFP vs. ChR2, p < 0.01 |