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. 2016 Mar 30;11(3):e0152386. doi: 10.1371/journal.pone.0152386

Fig 1. Expression analysis in HEK293 cells of commonly used expression tags.

Fig 1

(A-D) The expression levels were compared by western blot analysis detecting the double Strep II tag, which is present as an N-terminal tag on all proteins. (A) Different short arm laminin chains and netrins were transiently expressed and analyzed by western blot. (B) Different expression tags were transiently expressed and visualized by western blot. (C) Netrin-1 delta was fused to different expression tags. (D) Expression of netrin-4 delta in different vector systems was analyzed by western blot analysis. (Isom.: modified SlyD; mFc: Fc part from the mouse IgG protein; mFc-opt: codon optimized Fc part from the mouse IgG protein; ser alb: serum albumin; MBP: maltose binding protein; basic: without fusion protein).