Figure 1.

PI3K-C2α promotes localization of active Rab8a at the primary cilium. Immunofluorescence analysis of control and Sh1-IMCD3 cells transfected with either GFP-Rab8 or the constitutively active mutant GFP-Rab8^Q67L(green). Cells were starved for 24 hours and forming cilia were stained with anti-acetylated tubulin (red) and analyzed for the presence of Rab8. In control cells both the wild-type Rab8 and Rab8^Q67L were localized in cilia. On the contrary, in Sh1-IMCD3 cells, only the constitutively active Rab8^Q67L could be found at cilia. Bar=3 μm. PI3K-C2α, phosphoinositide 3-kinase-C2α; IMCD3, inner medullary collecting duct 3.