Figure 9.

Partial virtual nephron reconstructions are possible with clearing and 3D microscopy and illustrate most clearly the defect patterns in response to cisplatin. (A) Normal nephron showing glomerulus (green) with patent arterioles (red) and nearly complete coverage by capsular cuboidal cells (gray). (B) CP mouse nephron with normal-appearing glomerulus and arterioles, absent capsular cuboidal cells, but patent proximal tubule extending to medulla. (C) CP mouse nephron with absent capsular cuboidal cells and patent proximal tubule connection but a tubule that ends blindly before reaching medulla. The reduced brightness of tubules in parts B and C reflects reduced protein staining, suggesting cellular damage.