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. 2015 Aug 18;27(4):1076–1090. doi: 10.1681/ASN.2015010095

Figure 2.

Figure 2.

Transfer of WT BMDCs to S1pr3−/− mice induces kidney injury after IRI. (A) WT BMDCs treated with 100 ng/ml LPS for approximately 20 hours (WT BMDCs) or no cells (NCs; PBS) were injected (iv) 1 day before sham surgery or IRI into naïve WT and S1pr3−/− mice. Recipient mouse kidneys were exposed to 26 minutes of ischemia (or sham surgery in some cases) followed by 24 hours of reperfusion, and samples were collected 24 hours later (n=3–4). (B) H&E staining of kidney sections from the same mice; insets show a ×2.5 magnified image. (C) Naïve WT mice were injected (iv) with NCs, WT BMDCs, or S1pr3−/− BMDCs 1 day before kidney IRI (n=5–6 for IRI and n=2–3 for sham-operated mice). (D) H&E staining of kidney sections from the same mice. (E) WT naïve mice were injected (iv) with NCs, WT BMDCs, or S1pr3−/− BMDCs 7 days before kidney IRI (n=3). (F) H&E staining of kidney sections from the same mice. Values are mean±SEM. Scale bars, 100 μm. ***P<0.001. H&E, hematoxylin and eosin.