Figure 7.

Mice treated with S1pr3^−/− BMDCs have higher numbers of T_REGs and higher T_REG-DC interactions. (A) Spleen sections of liposome- or LC-treated mice subsequently treated (as in Figure 6) with no cells (NCs), WT BMDCs, or S1pr3^−/− BMDCs. Sections were labeled with antibodies for CD169 (red; macrophages), CD103 (green; DCs), or Foxp3 (magenta; T_REGs) or with DAPI (blue; nuclei). Regions of the spleen are labeled: MZ, white pulp (WP), and red pulp (RP). *Interactions of CD103⁺ and Foxp3⁺ cells. (B and C) Quantification of (B) CD103⁺ DC and T_REG (Foxp3⁺) interactions per white follicle and (C) T_REGs per follicle in the WP of liposome-treated mice. Values are mean±SEM (n=3–4 mice). Scale bar, 100 μm. *P<0.05; **P<0.01.