Figure 9.

S1pr3^−/− BMDCs or splenocytes induce tolerance in Balb/c mice and protect kidneys from ischemic injury. (A–F) Untreated C57BL/6 WT or S1pr3^−/− BMDCs (0.5×10⁶ iv) were adoptively transferred to naïve Balb/c mice 1 day before kidney IRI. (A) PCr (dashed line indicates mean value for sham-operated mice) and (B) H&E–stained kidney sections 24 hours after IRI. Total (C) CD45⁺ cells and (D) neutrophils (CD45⁺7AAD⁻CD11b⁺Ly6G^high) in kidney after IRI determined by flow cytometry. (E) Representative flow cytometry plots of kidney neutrophils. (F) Total spleen T_REGs (CD4⁺Foxp3⁺) determined by flow cytometry. (G) Representative flow cytometry histograms of Foxp3⁺ cells derived from gating on CD4⁺ or CD45⁺ spleen cells. (H) PCr (24 hours after IRI) in Balb/c mice treated with C57BL/6 WT or S1pr3^−/− BMDCs 7 days before kidney IRI. (I and J) Splenocytes were harvested from Balb/c mice 2 days after injection with WT (C57BL/6) or S1pr3^−/− BMDCs and transferred (5.0×10⁶ iv) to Balb/c recipient mice 1 day before IRI. (I) PCr was measured 24 hours after IRI. (J) H&E–stained kidney sections from the same mice. Values are mean±SEM (n=3–4 mice). NC, no cell. Scale bar, 100 μm. *P<0.05; **P<0.01. H&E, hematoxylin and eosin.