Figure 5.

TM208 and Tam increased the expression and activity of EST in MCF-7 xenograft tumors. (A) Western blot results showed that TM208 and Tam induced the protein expression of intratumoral EST. The expression of β-actin was included as a loading control. (B) EST immunostaining on tumor paraffin sections also demonstrated the induction of EST by TM208 or Tam. The brown staining intensity represents the expression of EST in the cytosol of tumors, and the blue staining represents the cell nucleus. The original magnification is 100× for all panels. (C) TM208 and Tam significantly increased the enzyme activity of EST in tumors. (D) Compared with the corresponding control group at different times, the mRNA expression of EST in tumors was significantly induced by the continuous administration of TM208 (150 mg·kg⁻¹·d⁻¹). VEH, vehicle. ^bP<0.05, ^cP<0.01 vs vehicle.