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. 2016 Apr 1;27(7):1131–1142. doi: 10.1091/mbc.E15-09-0648

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© 2016 Ono and Ono. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Phosphomimetic MLC-4 mutant impairs ovulation. Dissected gonads from mlc-4/+ worms expressing GFP–MLC-4(WT) (ML1150; A, C, E, G) or GFP–MLC-4(DD) (ML1151; B, D, F, H) were stained for GFP (A, B), F-actin (C, D), and DNA (E, F). Regions of the myoepithelial sheath. Large aggregates of DNA in F indicate the presence of endomitotic oocytes. Arrows indicate locally concentrated GFP–MLC-4(DD) and F-actin, suggesting that the constitutively active MLC-4 mutant induced contraction of the actomyosin networks.