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. 2016 Feb 10;107(3):359–368. doi: 10.1111/cas.12859

Figure 2.

Figure 2

I‐branching N‐acetylglucosaminyltransferase (GCNT2) expression controls prostate cancer cell invasion. (a) Messenger RNA expression of GCNT2 was determined in DU145 cells using quantitative PCR. GCNT2 expression levels were normalized to those of human G3PDH. Clone 3 and Clone 4 (DU145GCNT2KD3 and DU145GCNT2KD4) showed >70% inhibition of GCNT2 expression. (b) Protein expression of GCNT2 was determined in DU145‐derived cells using Western blotting. The GCNT2 expression level was lower in DU145GCNT2KD3 and 4 than in DU145NC. (c) In vitro cell proliferation was similar in DU145GCNT2KD3 and 4 cells at day 7, and strongly reduced cell migration (d) and invasion (e) was observed in Transwell assays. Assays were carried out in triplicate. *P < 0.05.