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Epithelial–mesenchymal transition is induced by RAP80 knockdown. (a) Control and RAP80 knockdown HeLa cell lysates subjected to immunoblotting analysis using anti‐RAP80, vimentin, E‐cadherin, N‐cadherin, c‐myc and succinate dehydrogenase (SDHA) as loading control.31 (b) Total RNA extracted from control and RAP80 knockdown Hela cells subjected to RT‐PCR with vimentin specific RT‐PCR primers. Averages of three independent experiments were presented with error bars.
