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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Brain Res. 2015 Sep 30;1638(Pt A):42–56. doi: 10.1016/j.brainres.2015.09.020

Figure 2. Diagram of iPSC differentiation and potential endpoints at the NSC stage.

Figure 2

Human HD iPSCs were differentiated into NSCs, then neurons and glia. At the NSC stage, we observed disease-associated phenotypes. When stressed (change from full medium to basic medium without growth factors) NSCs derived from HD iPSCs demonstrated upregulation of the N-terminal 513aa Htt fragment (recognized by neoHtt513 antibody) and cleaved caspase-3 and downregulation of BDNF and phalloidin labelled F-actin.